Donkey Secondary Antibodies
Biotium Cytochrome C (Mitochondrial Marker)(CYCS/3128R), CF647 conjugate, 0.1mg/mL
Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9
Biotium TAG-72 / CA72.4 (Tumor-Associated Glycoprotein)(rB72.3), CF647 conjugate, 0.1mg/mL
Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluoresc
Biotium PAX8 (Renal Cell Marker)(rPAX8/1492), CF647 conjugate, 0.1mg/mL
Recognizes a protein of 62 kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors. Primary antibodies are available purified, or with a
Bioassay Systems EnzyChrom™ L-Carnitine Assay Kit, For determination of L-Carnitine in urine, serum, plasma, cell lysates, tissue lysates and food products (e.g. milk).
L-CARNITINE is found in most mammals, plants, and some bacteria. L-Carnitine’s most crucial role is in the transport of long-chain fatty acids from the cytosol into the mitochondria for fatty acid oxidation. BioAssay Systems’ method provides a simple and high-throughput assay for measuring L-Carnitine. In this assay, a multistep reaction produces H2O2 which reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the L-Carnitine concentration in the sample.
Bioassay Systems Saccharide Removal Kit, For rapid removal of interfering saccharides (e.g. glucose, sucrose) from samples such as culture media, wines, beers, fermented teas.. Treated samples can be directly used for ethanol determination with DIET-500 assay kit.
SACCHARIDES such as glucose and sucrose are known to interfere with the QuantiChrom™ Ethanol Assay (catalog# DIET-500). BioAssay Systems has developed a rapid procedure for the complete removal of saccharides by co-precipitation with alkaline cupric and calcium ions. The treatment takes as little as 5 min and removes >99.9% saccharide from a sample.
Biotium ORC1(7F6/1), CF647 conjugate, 0.1mg/mL
The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells. Primary antibodies are available purified, or with a selection of flu
Biotium EGLN1 / PHD2(366G/76/3), CF647 conjugate, 0.1mg/mL
This antibody recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for det
Immunology Consultants Affinity Purified Donkey anti-Rabbit IgG H+L Antibody - Highly Cross-Adsorbed, 1.0 mg
Donkeys were immunized with highly purified rabbit IgG h+l and resulting antiserum was collected. Antibody was solid phase adsorbed using bovine, chicken, goat, guinea pig, horse, hamster, human, mouse, rat and sheep immunosorbents to remove cross reactive antibody. Specific antibody was immunoaffinity purified off a rabbit IgG containing immunosorbent. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG. This antibody will react with rabbit IgG and with light chains common with other rabbit immunoglobulins as determined by ELISA and IEP techniques. It has less than 1% cross reactivity with bovine, goat, guinea pig, horse, hamster, human, mouse, rat and sheep IgG and chicken IgY. This antibody is suitable for blotting, ELISA and IHC applications. This antibody may cross react with IgG from other species. Optimal working dilutions should be determined experimentally by the investigator.
Biotium NKX3.1 (Metastatic Prostate Adenocarcinoma Marker)(NKX3.1/3348), CF647 conjugate, 0.1mg/mL
NKX3. 1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21. 2, which frequently undergoes a loss of heterozygosity. NKX3. 1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3. 1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3. 1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note
Biotium PAI-RBP1 / SERBP1 / SERPINE1(SERBP1/3493), CF647 conjugate, 0.1mg/mL
PAI-RBP1 (plasminogen activator inhibitor 1 RNA-binding protein), also known as SERBP1 (SERPINE1 mRNA-binding protein 1), CGI-55, CHD3IP (chromodomain helicase DNA binding protein 3 interacting protein), HABP4L or PAI-RBP1, is a membrane-associated protein that localizes to the nucleus, the perinuclear region of the cytoplasm and the plasma membrane. PAI-RBP1 is believed to play a role in the regulation of mRNA stability, as it specifically binds to the CRS (cyclic nucleotide-responsive sequence) motif of the PAI-1 mRNA and acts to stabilize the mRNA and regulate its expression. In addition, PAI-RBP1 interacts with Mi2- and may be involved in chromatin remodeling. PAI-RBP1 also interacts with PGRMC1 and participates in the transduction of Progesterone's antiapoptotic action in ovarian cell types. The gene encoding PAI-RBP1 is overexpressed in ovarian cancer, suggesting a possible role for PAI-RBP1 in tumorigenesis and tumor metastasis. Primary antibodies are available purifie
Biotium Primary Antibody Mouse Fab kappa N2507.MFAB CF647 1/EA
Alpaca Anti-Mouse (Fab) Kappa rVHH (N2507 MFAB) is a recombinant Alpaca VHH antibody that recognizes the mouse kappa light chain and is validated for IF flow and WB Far-red fluorescent STORM-compatible CF647 dye has Ex/Em at 650/665 nm Unit size 500 uL at 0 1 mg/mL
Biotium Primary Antibody CD81 NA ExoBrite 560 585 1/EA
ExoBrite 560/585 CD81 anti-mouse Flow Antibody is optimized for detection of the exosome marker CD81 in isolated exosomes by flow cytometry The conjugate has Ex/Em at 562/583 nm for detection in the PE channel off the yellow laser line Recommended for instruments optimized for small particle flow 100 tests
Jackson Immuno Research Labs DONKEY ANTI-MOUS 6NM GOLD CONJ
6 nm Colloidal Gold-AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Shp Sr Prot) (EM Grade)
Electron Microscopy Sciences Aurion ImmunoGold Donkey-anti-Rabbit IgG (H&L), EM-grade 10nm 2.5 ML
It is impossible to perform a double labeling experiment in combination with, for instance a mouse primary antibody, since applicable secondary antibodies are either raised in rabbit or goat. To overcome this difficulty the Aurion Series of Secondary ImmunoGold conjugates is based on antibodies raised in Donkey, including Donkey-anti-Goat. It is then known that mixing Donkey-anti-Goat and Donkey-anti-Mouse provides a successful solution.
Particle size: EM-grade 10nm
Biolegend Cyanine3 Donkey anti-rabbit IgG (min. x-reactivity)
Cyanine3 Donkey anti-rabbit IgG (min. x-reactivity) [Poly4064]; Isotype: Donkey Polyclonal Ig; Reactivity: Rabbit; Apps: ICC; Size: 100 ug